Research Articles
Optimizing Protein Concentration Measurement: A Guide to Enhanced Accuracy for Reliable Research and Development
Accurate protein quantification is a foundational pillar of research and drug development, yet it is challenged by the lack of a universal gold standard and numerous potential interferences.
Addressing Proteolysis in Protein Purification: From Workflow Challenges to Targeted Degradation Technologies
This article provides a comprehensive analysis of proteolysis in protein workflows, addressing both the challenge of unwanted protein degradation during purification and the opportunity of intentional proteolysis for therapeutic purposes.
Strategic Approaches to Reduce Endotoxin Contamination in Recombinant Proteins: From Prevention to Removal
This comprehensive article provides researchers, scientists, and drug development professionals with advanced strategies for controlling endotoxin contamination throughout recombinant protein production.
Optimizing Protein Homogeneity and Dispersity: A Comprehensive Guide for Reproducible Research and Drug Development
Achieving optimal protein homogeneity and dispersity is a critical, yet often challenging, prerequisite for reproducible research in biochemistry, structural biology, and drug development.
Strategies for Enhancing Marginal Protein Stability in Heterologous Expression Systems
This article provides a comprehensive overview of advanced strategies for improving the marginal stability of recombinant proteins, a critical bottleneck in heterologous expression for biomedical research and drug development.
Chaperone-Assisted Protein Refolding: Protocols, Mechanisms, and Clinical Applications
This article provides a comprehensive guide to chaperone-assisted protein refolding, bridging foundational principles with practical laboratory applications.
Essential Quality Control for Recombinant Proteins: A Practical Guide to Improve Research Reproducibility
This article provides a comprehensive framework for implementing minimal quality control (QC) tests for recombinant protein samples, targeting researchers, scientists, and drug development professionals.
Quantifying Liposomal Protein Encapsulation: Advanced Methods for Efficiency Analysis and Optimization
Accurate determination of encapsulation efficiency (EE) is a critical quality attribute for developing effective liposomal protein formulations.
Evolution-Guided Atomistic Design: The Next Frontier in Protein Optimization and Therapeutic Development
This article explores the transformative methodology of evolution-guided atomistic design, a powerful computational strategy that synergizes analysis of natural evolutionary sequences with atomic-level physics-based calculations to optimize protein stability and...
Absolute Protein Oligomeric State Characterization: A Comprehensive Guide to SEC-MALS
This article provides a comprehensive overview of Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) for the absolute characterization of protein oligomeric states.